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Cells were cultured at 37 C in Dulbeccos modified Eagles medium (DMEM) + GlutaMax 1965 (Gibco) supplemented with 10% fetal bovine serum and 1% penicillinstreptomycin in a humidified 10% CO 2 incubator
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The study analyzed fecal and urine samples to assess gut microbiota composition (via 16S rRNA sequencing and FISH) and metabolic profiles (via 1 H-NMR spectroscopy), with behavioral questionnaires and GI symptom diaries
Cagrilintide. National Center for Biotechnology Information
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