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The LR3 modification provides a 20-30 hour half-life (vs minutes for native IGF-1), reduced IGFBP binding, and approximately 2-3x potency
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL5
LC-MS molecular weight confirmation: Mass spectrometry should confirm that the molecular weight matches the expected tripeptide-copper complex structure, approximately 340 Daltons for the GHK tripeptide, with copper binding verified
Asian J Pharma Clin Res 5(2):1518 Islam MT, Ali ES, Uddin SJ et al (2018) Phytol: a review of biomedical activities
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