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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
Kaneko N, Nilsen TO, Tanaka H, Hara A, Shimizu M (2020) Intact rather than total circulating insulin-like growth factor binding protein-1a is a negative indicator of growth in masu salmon
ClinicalTrials.gov (NCT07092605, July 30, 2025) Here are a few trials that support use of GLP-1s for other than diabetes and weight loss
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