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Briefly, HEK-293T (American Type Culture Collection, ATCC-CRL-3216) cells were cultured in DMEM supplemented with 10% FBS and antibiotics (penicillinstreptomycin, Gibco, 11528876)
Concentration is simply total mass divided by total volume: 70 mg 3 mL = 23.33 mg/mL Every 1 mL now contains 23.33 mg of blended peptide
doi: 10.1038/nature07039
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