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Cells are gated on CCR2-tdTomato and CX3CR1 to identify microglia (CCR2-tdTomato CX3CR1 + P2RY12 + ), CAMs/cDC2s (CCR2-tdTomato CX3CR1 + P2RY12 ), Mdcs (CCR2-tdTomato + CX3CR1 + )
The GSH:GSSG ratio reflects cellular redox balance healthy cells maintain ratios above 100:1, meaning reduced glutathione vastly outnumbers oxidized glutathione
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