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FOXO transcription factors at the interface between longevity and tumor suppression

Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

The shortest answer to what is GHK-Cu is this: it is a copper-binding tripeptide that your body produces naturally and uses as a repair signal