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To calibrate the measurement baseline, a lipid film composed of 20:1PC, 2.5 mol% Ni-NTA, 0.15mol% DOPE-LissRhod B, and 1 mol% TopFluor-Cholesterol was rehydrated with MQ water, sonicated at low power (20% of max), added to MOPS buffer (final concentration, 50mM MOPS pH 7.4, 100mM NaCl), and filtered through a 200nm PTFE filter (Millipore)
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Francesca Santacatterina Publications Department 28.07.2022 Reference: HPRA
This means lighter individuals experience higher peak concentrations relative to their body weight, which may explain why they often report more intense side effects and a stronger wearing-off effect
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