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As discussed above, LPL activity is regulated by multiple physiological factors and daily circumstances such as exercise and fasting
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To elucidate the influence of GLP-1R upregulation in LS GLP-1R neurons on food intake, we used a specific AAV vector encoding Cre-inducible mouse GLP-1Rs, complemented with a hemagglutinin (HA) epitope tag, for targeted injection into the dLS of GLP-1R-ires-Cre mice (Supplemental Figure 5A)
