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Lyophilized (unopened): Store at 20 C (4 F) or colder (80 C optimal) in dry, dark conditions for up to 12 months [4]
Applications Total Glutathione Colorimetric assay kit measures total, oxidized and reduced glutathione in wide range of samples such as blood, plasma, serum, cultured cells and tissues Estimating glutathione in cells is one of the ways to measure oxidative stress and this assay is also vital for mitochondrial toxicity screening GSH/GSSG ratio decreases in many pathological diseases such as cancer, diabetes, cardiovascular disorders, artherosclerosis, Parkinsons and Alzheimers 3, 4 and thus estimating glutathione is vital in this area of research
Splenocytes were washed with warm RPMI 1640, stained at 37 C for 30 min with Abs diluted in RPMI 1640, and washed with PBS
They act as antioxidants, protecting our cells from harm
