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Pinch a fold of skin, insert the needle at about a 45-degree angle, and inject the solution
Approximately 100 L of blood was then aseptically collected from the lateral tail vein using a 26-gauge needle attached to a heparinized 0.5 mL insulin syringe under direct supervision by experienced researchers
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The reaction was performed at 22 C for 20 min, and the absorbance at 546 nm was measured, using NaNO 2 solution as standard
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