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For a clinician-facing summary of timing and management considerations see this safety review

Mice were housed under standard laboratory conditions with controlled temperature and humidity, a 12 h light/dark cycle, free access to food and water, and environmental enrichment

(2) To remove hydrophobic substances, wash the column with 3-4 bed volumes of 70% ethanol or 2 bed volumes of 1% Triton X-100 followed by 5 bed volumes of PBS (pH 7.4) washed

Although GPX4 is the primary mechanism for inhibiting iron poisoning, studies have shown that cells deficient in GPX4 can still survive in the activated state of ferroptosis