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Its copper-dependent activity also helps stabilize enzymes responsible for tissue repair

Measurement of reactive oxygen species and lipid peroxidation Cells were incubated with 5 M BODIPY 581/591 C11 (Life technologies) for lipid peroxidation, or 5 M CM-H2DCFDA (Life technologies) for ROS in the dark for 30 minutes at 37 C

The glutathione concentration of unknown sample is measured by measuring the absorbance at 415 nm and comparing it with standard curve for GSSG, which is plotted new every time when glutathione quantification is done