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To create the tnaA mutant, the DNA fragment containing the kanamycin resistance cassette for homologous recombination was amplified by PCR using the primers F- tnaA -FRT and R- tnaA -FRT and the plasmid pKD4 as the template

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The benzyl alcohol inhibits the growth of most microorganisms, allowing the solution to be used for multiple reconstitutions from a single vial a critical advantage over single-use sterile water for researchers working with lyophilized compounds