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H 2 O 2 -treated zygotes had elevated levels of ROS, as well as increased oxidative damage, activation of the G2/M checkpoint, decreased cleavage and blastocyst rates, and increased rates of apoptosis

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)