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Luciferin detection reagent (LDR) (100 l) was added to all wells, briefly shaken and incubated for 15 min at room temperature and the luminescence measured using microplate imager (BioTek Cytation3)

Cells were then subjected to various treatments as outlined in Section 2.7

Clinical studies reveal that patients, on average, lost 15 to 20 percent of their body weight over 72 weeks, which could equate to 37 to 50 pounds for someone weighing 250 pounds

Reevaluation of glucose-dependent insulinotropic polypeptide (GIP) pharmacology in combination with glucagon-like peptide-1 (GLP-1) for metabolic diseases: Overview on biological GIP and GLP-1 effects at the organ/tissue level