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If this happens, the integrity of the compound is compromised, and for research purposes, it should ideally be discarded to avoid skewed results
The nuclei were counterstained using DAPI (Thermo Fisher Scientific), after which the stained sections were imaged with a confocal microscope (LSM880, Zeiss)
Permeabilization assays with 1 (in black), 5 (in yellow) and 10 mM (in brown) GSH measured in intervals of 2.5 minutes until 60 minutes
Furthermore, pupils and even teachers can telephone the centre to obtain information
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