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To generate G3BP-tdTomato knock-in U2OS cells that stably express WT or mutant forms of ASPL, HEK293T (CRL-3216) cells were first transfected with the N174 lentiviral vector (Addgene, #31822) containing different ASPL cDNAs along with packaging vectors PSPAX2 and PMD2.g using Lipofectamine 2000 (Thermo Fisher Scientific)
doi: 10.1210/endo-123-4-2009 19 WiceBMReedsDNTranHDCrimminsDLPattersonBWDunaiJet al
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Fardouly J, Vartanian LR
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