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An increased density implies a higher cell age as has been established by the decreased activities of a number of intracellular enzymes, measured in the lysate (hexokinase, aldolase, pyruvate kinase, glutamate-oxalacetate transaminase) (Table 1)

It is therefore suggesting that the slight change of relaxation observed in the aorta without endothelium ex vivo might be due to some mechanism unrelated to the direct nitric oxide stimulation which needs further investigation