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In rats assessed 24 weeks after ischemic injury, minocycline improved perfusion, reduced BBB permeability with higher levels of TJ proteins, and decreased the frequency of proinflammatory microglia, shifting their activation to an M2-like, or anti-inflammatory, phenotype with upregulation of transforming growth factor beta (TGF- ) and IL-10 and decreased TNF- and IL-1 (Yang et al., 2015)
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Nevertheless, despite demonstrating potent in vivo effects, neither study performed side-by-side dose-response benchmarking against clinical ACE inhibitors (e.g., lisinopril or captopril) under identical assay conditions
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