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Cell culture and treatment Immortalized human keratinocyte (HaCaT) cells (NE Fusenig, Heidelberg, Germany) were cultured in adipocyte medium (AM, high-glucose DMEM with 10% FBS and 1% P/S) at 37 C in a humidified incubator with 5% CO 2
We assessed keratin expression and found that the elevated expression of K16 and K17 diminished in both in vivo and in vitro experiments following SFN treatment
D., et al
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