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Human recombinant Trx1 (0.1 M) was prereduced in a 96-well microtiter plate for 30 min at 37 C in the presence of 50 nM TrxR and 0.25 mM NADPH in TE buffer (100 mM Tris, pH 7.5 and 10 mM EDTA) in final concentrations
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