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Prior to the ITC experiments, concentrated ShD14, ShHTL1, ShHTL4, and ShHTL7 were exchanged into a buffer consisting of 20 mM HEPES (pH 8.0) and 150 mM NaCl to remove dithiothreitol and were then adjusted to a final concentration of 150 M

Red blood cell storage: from genome to exposome towards personalized transfusion medicine

What has been slower to emerge is a framework that recognizes these not as independent problems but as coordinated consequences of a single underlying disruption: the withdrawal of hormonal signals that had, for decades, been quietly governing cellular energy regulation, inflammatory tone, and tissue maintenance throughout the body

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