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Whole-mount embryo immunostaining E9.5 embryos ( Trim37 ko1 and Trim37 cko ) were harvested, fixed in 4% paraformaldehyde at 4 C for 2 h, and washed twice with PBS

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While GLP-1 and GIP signalling primarily enhance insulin secretion and modulate appetite, glucagon receptor activation increases hepatic glucose output, stimulates lipolysis in adipose tissue, and promotes thermogenesis

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