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PBMCs were purified from other blood components and the ficoll histopaque by washing twice with 1x phosphate-buffered saline (PBS)
In vitro and ex vivo uptake of glutathione (GSH) across the intestinal epithelium and fate of oral GSH after in vivo supplementation
2015 Oct;47(10):2177-84
Then, a TPA-degrading strain Pseudomonas stutzeri TPA3 was employed for the biosynthesis of PHB, which was achieved by the integration of the phbCAB operon from Ralstonia eutropha
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