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Sucrose Gradient Sedimentation of the Cytosolic TuRC HEK293T cells, grown to confluence, were washed with HBS (50 mM HEPES, pH 7.4, and 150 mM NaCl) and were then lysed at 4C in HBS supplemented with 1 mM DTT, 1 mM EGTA, 1 mM MgCl 2 , 0.25 mM GTP, 0.5% Triton X-100, and the Protease Inhibitor Cocktail
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The AZIN2 protein localizes to the endoplasmic reticulum (ER) and the Golgi
If youre an athlete and often push your body to the limit, you likely suffer from an increased production of free radicals
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