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While prevalence data for subclinical gut overgrowth is difficult to quantify precisely because it is underdiagnosed functional medicine practitioners report that it is among the most common root-cause findings in patients presenting with chronic fatigue, irritable bowel syndrome, and treatment-resistant mood disorders

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After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)

Expert Rev Clin Pharmacol 6(3):323332 Uckert S et al (2001) Phosphodiesterase isoenzymes as pharmacological targets in the treatment of male erectile dysfunction