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Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40
Potassium replacement therapy should be administered if necessary (10, 13)
Gas, forming in intestines and pushing upwards, can be very troublesome
By targeting GLP-1, GIP, and glucagon pathways, the asset is designed to affect appetite, glycemic control, and energy metabolism
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