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Immunocytochemistry Anti-GFP (1:1000, Abcam) antibody was applied in blocking solution (PBS, 0.05% BSA, 2% FBS, 1% Triton X-100, and 0.1% saponin) for 2 h at room temperature
CGI, clinical global impression
Expected weight loss: 13 kg (mostly appetite-driven reduction) 1.0 mg Weeks 912 Maximum Ozempic dose and the midpoint of Wegovy titration
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