USA 107 , 2250522510 (2010)
Patients pursuing fat loss or building muscle should understand that peptides are not substitutes for lifestyle changes such as nutrition and physical activity

After blocking, the membranes were incubated overnight at 4 C with the following primary antibodies: PFKFB3 (1:2000, Proteintech, Wuhan, China, 13763-1-AP), NLRP3 (1:500, Novus, Littleton, CO, USA, NBP2-12446), ASC (1:1000, Cell Signaling Technology, Danvers, MA, USA, 67824s), Caspase-1 p20 (1:1000, Proteintech, Wuhan, China, 22915-1-AP), collagen I (1:2000, Proteintech, Wuhan, China, 14695-1-AP), -SMA (1:4000, Proteintech, Wuhan, China, 14395-1-AP), -actin (1:5000, Proteintech, Wuhan, China, 20536-1-AP), Pan-Kla (1:1000, PTM Biolabs, Hangzhou, China, PTM-1401RM), H4K5la (1:1000, PTM Biolabs, Hangzhou, China, PTM-1407RM), H4K12la (1:1000, PTM Biolabs, Hangzhou, China, PTM-1411RM), H3K18la (1:1000, PTM Biolabs, Hangzhou, China, PTM-1406RM), H4 (1:2000, PTM Biolabs, Hangzhou, China, PTM-1004), H3 (1:2000, PTM Biolabs, Hangzhou, China, PTM-1001), and p300 (1:1000, Cell Signaling Technology, Danvers, MA, USA, 67621)

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