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Western blotting Protein lysates were extracted from frozen RV tissue or cultured cardiomyocytes using RIPA buffer supplemented with protease and phosphatase inhibitors (Beyotime, China)
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The Conservative 16-Week Escalation For individuals with a history of sensitivity to GLP-1 medications, previous GI issues, or a general preference for caution, a slower escalation stretches the timeline to 16 weeks or longer
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